SAN DIEGO, Nov. 14, 2016 /PRNewswire/ -- Findings from a recent study show Kemin Industries' proprietary, spearmint-based cognitive performance ingredient, Neumentix™ Phenolic Complex K110-42, enhances rates of neurogenesis at physiologically relevant concentrations in rat primary hippocampal cells. Photo - http://photos.prnewswire.com/prnh/20161110/438081 The study, "The effects of a proprietary spearmint extract on neurogenesis in rat hippocampal neurons," was conducted at QPS in Grambach, Austria. Key findings on the novel mechanism of action were presented Saturday, November 12 at the Society for Neuroscience annual meeting, Neuroscience 2016, in San Diego. "This study was designed to test our hypothesis that neurogenesis is a mechanism of action for the clinically observed working memory improvements associated with Neumentix supplementation," said Laura Wonderling, Ph.D., research and development director for the Human Nutrition and Health division of Kemin. Primary hippocampal neurons were prepared from Sprague Dawley rats at embryonic day 18. The cells were treated with Neumentix at final concentrations of 10 nM, 100 nM, 1 µM, 100 µM rosmarinic acid (RA), vehicle or fibroblast growth factor (FGF) as a positive control, for a total of 48 hours. At the 24-hour mark, Bromdesoxyuridine (BrdU) was used to label proliferating cells. At the 48-hour mark, samples were stained with NeuN to label neurons and BrdU, and then stained with 4', 6-diamidino-2-phenylindole (DAPI) to label DNA. Proliferating neurons (BrdU, NeuN and DAPI positive) and total number of neurons (NeuN and DAPI positive) were determined with digital imaging. Analysis showed the neurons responded differently to various concentrations of Neumentix. Cultures treated in the lowest dose group (10 nM RA) displayed statistically significantly greater levels of neurogenesis than vehicle treated cultures. "The most effective concentration corresponds to levels detected in the plasma of human subjects two hours following oral supplementation with 900 mg Neumentix," said Wonderling. "This suggests the cognitive performance improvements observed in our previous clinical study could be due in part to Neumentix increasing rates of new neural growth in the hippocampus."