Pulse Biosciences, Inc., (NASDAQ:PLSE), a medical technology company developing a proprietary therapeutic treatment platform based on Nano-Pulse Stimulation (NPS), today announced the results of three preclinical studies that further validate the NPS technology and its potential role in novel immunotherapeutic approaches. The new data, which will be presented November 11-12 th at the Society for Immunotherapy of Cancer (SITC) Annual Scientific Meeting, demonstrate that NPS triggered immunogenic cell death (ICD) in three separate cancer cell lines and stimulated other beneficial anti-tumor immune responses in two in vivo studies. NPS is a non-thermal drug-free tissue treatment technology that involves the local application of a series of ultrashort electrical pulses, each lasting only hundreds of nanoseconds, directly to tissue through electrodes. In preclinical models, NPS has shown the potential to stimulate a signaling cascade within tumor cells that leads to ICD, or immunogenic apoptosis (programmed cell death). This process not only induces cell death in a natural way but also engages the immune system to clear the treated cells and enroll cytotoxic T cells to recognize and eliminate cells of the same tumor type. "These new data add to the growing body of scientific evidence supporting the use of NPS in the treatment of multiple tumor types," said Darrin Uecker, President and Chief Executive Officer of Pulse Biosciences. "We believe the immune response observed in these recent studies further indicate that NPS may have the ability to prime the immune system and may be effective, and potentially even synergistic, when used in combination with other immuno-therapeutics, with the distinct advantage of not adding any drug related toxicity." The three studies presented at SITC showed that NPS treatment resulted in potentially beneficial immune cell changes in both the primary tumor and untreated secondary tumor microenvironment. Specific findings are as follows:
- In one study presented, "Nanosecond pulsed electric field treatment of tumor cell lines triggers immunogenic cell death (ICD)," NPS induced three markers of ICD in three tumor cell lines, which the authors concluded could explain why in vivo NPS treatment has shown a vaccine-like effect that inhibits secondary tumor growth after subsequent challenges with tumor cells.
- In a second presented study, "Nanosecond pulsed electric field treatment of murine melanomas initiates an immune response and inhibits metastasis," mice were given a single melanoma tumor which was later either removed surgically or by NPS treatment. When subsequently challenged with intravenously injected melanoma cells, the NPS-treated mice exhibited fewer metastases than did the mice that had surgical removal, suggesting that the immune system had removed some of the melanoma cells from circulation in the NPS treated mice. This observation extends NPS's vaccination effect beyond secondary tumor challenge to circulating cancer cells.
- In the third study presented, "Adaptive immune response to Nano-Pulse Stimulation," NPS treatment of murine fibrosarcoma tumors significantly increased the infiltration of immune system cells into the treated primary tumor and 3 weeks later challenge tumors were completely rejected due to the production and increase in functionality of T cells.