Pacific Biosciences ( PACB) is feeling so confident about its offering, that its lifted its original filing from $200 million to $230 million. Biotech IPOs haven't received warm welcomes of late and Pacific Biosciences is in an increasingly crowded DNA sequencing field.

Plus, this market wants proven companies and Pacific Biosciences is all about potential. The company has already raised $400 million in venture capital money, while having lost $63 million for six months ending June 2010.

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