The layoff announcement Monday affects the company's self-driving and eats divisions, among others.
The letter from CEO Dara Khosrowshahi comes about four weeks after the company announced that it was laying off 435 employees across its engineering and product teams. The company said that this is the "last wave" of a process to streamline the company's operations.
"This time, ATG, Eats, Global Rides and Platform (Rides Ops, CommOps, Safety & Insurance, U4B, and Product Ops), Performance Marketing, and Recruiting have made changes," Khosrowshahi said. "Days like today are tough for us all, and the ELT and I will do everything we can to make certain that we won't need or have another day like this ahead of us."
The CEO closed the letter by saying that he will be at the company's "all hands" meeting on Tuesday to answer employee questions, CNBC reported.
Uber shares were rising 4.15% to $31.38 on Monday.