The Dip And Read(TM) Anti-FLAG Biosensor For Use On ForteBio's Octet(R) And BLItz(TM) Instrumentation Systems Enables Researchers To Quickly And Easily Detect FLAG-tagged Recombinant Proteins For Their Quantitation And Kinetic Characterization.
ForteBio, a division of Pall Life Sciences and a leading supplier of label-free technology that accelerates the development of biotherapeutic and pharmaceutical products, today announced the launch of its new Dip and Read™ Anti-FLAG Biosensor for use on the company’s Octet® and BLItz™ instrumentation systems. The new biosensor enables researchers to quickly and easily detect FLAG-tagged recombinant proteins for their quantitation and kinetic characterization, which are key steps in drug development.
“FLAG is a commonly used affinity peptide tag to enable protein detection and purification,” said Christopher Silva, vice president of marketing for Pall's ForteBio division. “However, traditional methods for evaluating FLAG-tagged proteins rely on time-consuming, analytical methods, which can slow down drug discovery and development. Our new Anti-FLAG Biosensor, together with the BLItz instrument platform’s ease of use or the Octet system’s high throughput, is designed to greatly accelerate laboratory workflow, reduce time to results and increase cost-efficiency.”
The biosensor is pre-immobilized with Sigma-Aldrich’s well-established, high-affinity ANTI-FLAG® M2 antibody, which has been cited in more than 30,000 journal articles over the last two decades. The high specificity of the antibody-based biosensor enables the direct quantitation of FLAG-tagged analytes in crude lysates, column eluents, cell lysates and cell culture supernatants.
Mr. Silva added that the new biosensor is intended to address strong demand for direct capture of FLAG-tagged proteins among ForteBio’s Octet customers. It also should further broaden the appeal of the BLItz instrument platform – which enables label-free analysis of proteins using small sample volumes on a benchtop instrument – among academic researchers. “We are now pleased to offer our customers biosensors for the three most widely used peptide tags – HIS, GST and FLAG – among pharmaceutical and biotechnology scientists.”
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