CARLSBAD, Calif., Dec. 19, 2012 /PRNewswire/ -- Life Technologies Corporation (NASDAQ: LIFE) announced today a new 400 base-pair sequencing kit for the Ion PGM™ Sequencer. The kit produces reads 60 percent longer than comparable high-throughput benchtop sequencers, generating more complete bacterial de novo assemblies with longer contiguous sequences. To see a video about the 400 bp kit and other new products, click here.
"Ion Torrent's long, accurate 400 base pair reads enable excellent bacterial de novo assembly," said Professor Dag Harmsen, Head of Research, Department of Periodontology, University of Muenster, who used the Ion PGM™ Sequencer to help crack the E. coli outbreak in Germany in 2011. "Paired-end sequencing is not required to get the best results."
The Ion PGM™ sequencing 400 kit is ideal for applications that require capturing all the genetic variation in a long exon on a single read. For instance, long reads are critical for researchers trying to interpret genetic data from human leukocyte antigen (HLA) genes or 16S ribosomal genes.
"Sequencing the V1-V2 region of the bacterial 16s rRNA gene would not be possible on the Ion PGM™ without the longer 400 base pair reads," said Dr. George Watts, Research Assistant Professor and Co-Director, Genomic Shared Service, University of Arizona Cancer Center. "Using 400 base pair reads allows us to increase the information density of the amplicons and better define the constituents of complex microbial populations."Ion Torrent continues to see rapid increases in accuracy on its Ion PGM™ and Ion Proton™ Sequencers as a result of consistent improvements in its algorithms. The new Torrent Suite v3.4 incorporates algorithmic improvements in both raw accuracy and consensus variant calling. These improvements enable both the Ion PGM™ and Ion Proton™ semiconductor sequencing platforms to routinely achieve greater than 99.996 percent consensus accuracy.