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American CareSource Announces First Quarter 2011 Earnings Conference Call

American CareSource Holdings, Inc. (NASDAQ: ANCI) today announced that it will release its financial results for the first quarter ended March 31, 2011, after the close of U.S. financial markets on Thursday, May 5, 2011. An accompanying conference call hosted by David Boone, President and Chief Executive Officer, and Matthew Thompson, Chief Financial Officer to discuss the results will be held at 10:30 a.m. ET, on Friday, May 6, 2011.

Conference dial-in: (888) 254-3563 International dial-in (913) 312-0977 Conference ID: 3928563 Webcast: http://ir.anci -care.com/events.cfm

An online audio replay of the conference call will be available one hour following the conclusion of the live broadcast on the company's website, www.anci-care.com, for one year.

About American CareSource Holdings, Inc.

American CareSource Holdings, the first national, publicly traded ancillary care network services company, offers a comprehensive national network of over 5,000 ancillary service providers at more than 36,000 sites through its subsidiary, Ancillary Care Services. Ancillary Care Services provides ancillary health care services through its network that offers cost effective alternatives to physician and hospital-based services. This market is estimated at $574 billion and has grown to over 20% of total national health expenditures. These providers offer services in 31 categories including laboratories, dialysis centers, free-standing diagnostic imaging centers, non-hospital surgery centers, as well as durable medical equipment such as orthotics and prosthetics.

The Company's ancillary network and management provides a complete outsourced solution for a wide variety of health care payors and plan sponsors including self-insured employers, indemnity insurers, preferred provider organizations, health maintenance organizations, third party administrators and both federal and local governments. For additional information, please visit www.anci-care.com.

ANCI-G

Safe Harbor Statement Under the Private Securities Litigation Reform Act of 1995:

Any statements that are not historical facts contained in this release, including with respect to the Company’s plans, objectives and expectations for future operations, projections of the Company’s future operating results or financial condition, and expectations regarding the health care industry and economic conditions, are forward-looking statements. Substantial risks and uncertainties could cause actual results to differ materially from those indicated by forward-looking statements, including, but not limited to, changes in national health care policy, regulation, general economic conditions, demand for ancillary services, pricing, competition, market acceptance/preference, the Company’s ability to integrate with its clients, changes in the business decisions by key clients or consolidation in the industry affecting them, the Company’s inability to attract or maintain providers or clients or to manage growth, implementation and performance difficulties, and other risk factors detailed from time to time in the Company’s periodic filings with the Securities and Exchange Commission. Except as otherwise required by law, the Company undertakes no obligation to update or revise these forward-looking statements.

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